This manual consists of several chapters that deal with the techniques involved in the study of aquatic pathogens that cause infections, especially in fish. It covers a wide range of basic and advanced techniques associated with research on the isolation and identification of bacterial, viral, and fungal pathogens, and probiotic bacteria. In addition, it addresses the treatment of pathogens using seaweed extracts, medicinal plant extracts, and actinomycetes. The knowledge and information shared in this manual provides information on the various protocols to be followed while performing the experiments. The editors are extremely grateful to each author or team of authors who found time to write a comprehensive chapter based on their expertise in various protocols. These protocols covered in this manual are widely followed by researchers and, therefore, will be extremely useful. Post-graduate students, research scholars, postdoctoral fellows, and teachers belonging to different disciplines in microbiology, biotechnology, and marine science are the targeted readers of this manual. Reading this manual will kindle further discussions among researchers working in aquaculture, biotechnology, microbiology, and other related subjects, thus widening its broader scope. Researchers will gain more knowledge of the information shared here on various protocols.
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